LXR Agonist Increases ApoE Secretion from HepG2 Spheroids
Author Information
Author(s): Kurano Makoto, Iso-O Naoyuki, Hara Masumi, Ishizaka Nobukazu, Moriya Kyoji, Koike Kazuhiko, Tsukamoto Kazuhisa
Primary Institution: The University of Tokyo
Hypothesis
The study investigates the effect of the LXR agonist TO901317 on hepatic apoE production in HepG2 cells cultured in spheroid form.
Conclusion
LXR activation enhances the expression of hepatic apoE and alters the lipoprotein particles produced from differentiated hepatocyte-derived cells.
Supporting Evidence
- Spheroid HepG2 cells secreted more albumin and apolipoproteins than monolayer HepG2 cells.
- LXR activation with TO901317 significantly increased apoE protein secretion from spheroid HepG2 cells.
- The production of apoE-rich large HDL particles was enhanced with LXR activation.
Takeaway
The study found that a special drug can help liver cells make more of a protein called apoE, which is important for fat metabolism.
Methodology
The study used HepG2 cells cultured in spheroid form to investigate the effects of the LXR agonist TO901317 on apoE production and lipoprotein secretion.
Limitations
The study primarily uses a cell line model, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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