Selenium effectively inhibits 1,2-dihydroxynaphthalene-induced apoptosis in human lens epithelial cells through activation of PI3-K/Akt pathway
2011
Selenium Protects Human Lens Cells from Apoptosis
publication
Evidence: moderate
Author Information
Author(s): Zhu Xiangjia, Guo Kun, Lu Yi
Primary Institution: Fudan University
Hypothesis
Is the activation of the PI3-K/Akt pathway necessary for selenium to protect human lens epithelial cells from apoptosis induced by 1,2-dihydroxynaphthalene?
Conclusion
Selenium protects human lens epithelial cells from apoptosis induced by 1,2-dihydroxynaphthalene through the activation of the PI3-K/Akt pathway.
Supporting Evidence
- Sodium selenite increased cell viabilities and prevented apoptosis.
- Activation of the PI3-K/Akt pathway was necessary for selenium's protective effect.
- LY294002, a PI3-K/Akt inhibitor, blocked the protective effect of selenium.
Takeaway
Selenium helps keep eye cells healthy and prevents them from dying when they are stressed by harmful substances.
Methodology
Cell viability was assessed using CCK-8 and trypan blue exclusion assays, and apoptosis was evaluated by flow cytometry.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
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