Gene transfer and expression in human neutrophils
Author Information
Author(s): Jennifer L. Johnson, Beverly A. Ellis, Daniela B. Munafo, Agnieszka A. Brzezinska, Sergio D. Catz
Primary Institution: The Scripps Research Institute
Hypothesis
Can exogenous gene expression be achieved in human neutrophils using nucleofection?
Conclusion
The study demonstrated that cDNA transfer and expression of exogenous protein in human neutrophils is compatible with cell viability.
Supporting Evidence
- Neutrophils transfected by nucleofection are functional and can respond to stimuli.
- Transfection efficiency was observed to be 0.4 to 1%.
- Transfected neutrophils maintained their ability to undergo phagocytosis.
Takeaway
Scientists found a way to put new genes into human neutrophils without hurting the cells, which helps them study how these genes work.
Methodology
Neutrophils were transfected using nucleofection and analyzed for gene expression and functionality.
Limitations
The relatively low efficiency of transfection/expression restricts subsequent analysis to experiments that use single cells.
Participant Demographics
Human neutrophils isolated from healthy donors.
Digital Object Identifier (DOI)
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