Porphyromonas gingivalis short fimbriae are regulated by a FimS/FimR two-component system
2007
Regulation of Porphyromonas gingivalis Short Fimbriae by FimS/FimR System
publication
Evidence: moderate
Author Information
Author(s): Wu Jie, Lin Xinghua, Xie Hua
Primary Institution: School of Dentistry, Meharry Medical College
Hypothesis
Does the FimS/FimR two-component system regulate the expression of the mfa1 gene in Porphyromonas gingivalis?
Conclusion
FimR directly binds to the promoter region of the mfa1 gene, acting as a positive regulator of its expression.
Supporting Evidence
- FimR is a transcriptional activator of the mfa1 gene.
- Expression of mfa1 is repressed in the fimR mutant.
- FimR does not bind to the fimA promoter region.
Takeaway
This study shows that a protein called FimR helps control how another protein, Mfa1, is made in a bacteria that can cause gum disease.
Methodology
The study involved constructing a fimR mutant and analyzing gene expression using real-time PCR and western blotting.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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