The Effect of Purified Opharin Isolated from the Venom of King Cobra (Ophiophagus hannah) in Modulating Macrophage Inflammatory Responses and Vascular Integrity
2024

Effects of King Cobra Venom Protein on Inflammation and Blood Vessel Integrity

publication Evidence: moderate

Author Information

Author(s): Lertwanakarn Tuchakorn, Reyes Armando, Salazar Emelyn, Barrientos Martha, Sanchez Elda E., Suntravat Montamas

Primary Institution: Kasetsart University, Thailand; Texas A&M University-Kingsville, USA

Hypothesis

This study investigates the biological effects of Opharin, a protein from king cobra venom, on inflammatory responses and vascular integrity.

Conclusion

Opharin significantly increases inflammatory cytokine production and vascular permeability, suggesting its role in the severe effects of king cobra envenomation.

Supporting Evidence

  • Opharin increased levels of inflammatory cytokines IL-1β, IL-6, IL-8, TNF-α, and IL-10 in macrophages.
  • In vivo studies showed that Opharin increased vascular permeability by 26% compared to the control group.
  • The study utilized a two-step chromatography process to purify Opharin from king cobra venom.

Takeaway

The study found that a protein from king cobra venom makes the body react more strongly to inflammation and can cause blood vessels to leak, which is important for understanding snakebite effects.

Methodology

The study involved purifying Opharin from king cobra venom and testing its effects on inflammatory cytokine production in macrophages and vascular permeability in mice.

Limitations

Further research is needed to fully understand the pharmacological and toxicological effects of Opharin and other venom components.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.3390/toxins16120550

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