Thiamine Pyrophosphate in Mammalian Peroxisomes
Author Information
Author(s): Fraccascia Patrizia, Sniekers Mieke, Casteels Minne, Van Veldhoven Paul P
Primary Institution: Katholieke Universiteit Leuven
Hypothesis
This study was undertaken to get a better insight into the transport of thiamine and its phosphate esters across the peroxisomal membrane.
Conclusion
Mammalian peroxisomes contain TPP, but thiamine must enter the peroxisome already pyrophosphorylated.
Supporting Evidence
- TPP was found in peroxisomes for the first time.
- No thiamine pyrophosphokinase activity was detected in peroxisomes.
- TPP concentration in peroxisomes was estimated to be higher than in the cytosol.
Takeaway
The study found that a vitamin called thiamine pyrophosphate is present in tiny parts of cells called peroxisomes, but it doesn't get made there; it has to come in already prepared.
Methodology
The presence of TPP was measured in purified rat liver peroxisomes using HPLC coupled with fluorimetry.
Limitations
The study does not identify a specific transporter for TPP in peroxisomes.
Participant Demographics
Male Wistar rats and Swiss Webster mice were used in the study.
Digital Object Identifier (DOI)
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