SNPmplexViewer: A Cost-Effective Tool for DNA Traceability
Author Information
Author(s): Yanir Seroussi, Andrey Shirak, Eyal Seroussi
Primary Institution: Monash University
Hypothesis
Can SNPmplexViewer reduce the cost of DNA-based traceability in cattle by embedding size standards in trace files?
Conclusion
SNPmplexViewer enables cost-effective genotyping of SNaPshot reactions without fluorescent size standards, improving software performance.
Supporting Evidence
- SNPmplexViewer allows for the analysis of trace files without the need for expensive fluorescent size standards.
- The tool improves the performance of genotyping software by providing normalized trace outputs.
- SNPmplexViewer is available online for free, making it accessible for researchers.
Takeaway
SNPmplexViewer is a free tool that helps scientists analyze DNA traces more cheaply by using a computer program to add important information to the data.
Methodology
The tool uses a Perl script to analyze DNA trace files, normalizing and aligning data to embed size standards.
Limitations
The algorithm may not efficiently align traces from microsatellites due to their complex stutter patterns.
Digital Object Identifier (DOI)
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