Study of Oxidative Stress in Obese Rats
Author Information
Author(s): Noeman Saad A, Hamooda Hala E, Baalash Amal A
Primary Institution: Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt
Hypothesis
The study aims to investigate the effects of a high fat diet on oxidative stress markers in the liver, heart, and kidney of rats.
Conclusion
High fat diet-induced obesity leads to increased oxidative stress in the liver, heart, and kidneys, reducing antioxidant enzyme activities and glutathione levels.
Supporting Evidence
- Obese rats showed significant increases in MDA and PCO levels in liver, heart, and kidney tissues.
- Antioxidant enzyme activities were significantly reduced in the tissues of obese rats.
- GSH levels decreased in liver and kidney tissues of obese rats, while it increased in heart tissues.
Takeaway
Feeding rats a high fat diet makes them gain weight and harms their organs by increasing harmful substances and decreasing protective ones.
Methodology
Sixty rats were divided into two groups: one received a high fat diet for 16 weeks, while the other received a normal diet. Various biochemical assays were performed on blood and tissue samples.
Potential Biases
Potential bias may arise from the specific dietary composition and the controlled environment in which the rats were kept.
Limitations
The study was conducted on a specific strain of rats, which may limit the generalizability of the findings to other species or humans.
Participant Demographics
Sixty white albino rats, both males and females, weighing 150-200 g.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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