Selection of a set of reliable reference genes for quantitative real-time PCR in normal equine skin and in equine sarcoids
2006
Choosing Reliable Reference Genes for Equine Skin Studies
Sample size: 16
publication
Evidence: moderate
Author Information
Author(s): Bogaert Lies, Van Poucke Mario, De Baere Cindy, Peelman Luc, Gasthuys Frank, Martens Ann
Primary Institution: Ghent University
Hypothesis
The study aims to establish a reliable set of reference genes for normal equine skin and equine sarcoids.
Conclusion
TUBA1, ACTB, and UBB are recommended as reference genes for normal equine skin, while B2M, ACTB, and UBB are best for equine sarcoids.
Supporting Evidence
- The study analyzed 6 commonly used reference genes in equine skin and sarcoids.
- GeNorm software was used to assess gene expression stability.
- The results indicated that using multiple reference genes improves normalization accuracy.
Takeaway
The study found the best genes to use for comparing skin samples from healthy horses and horses with skin tumors.
Methodology
The study used real-time quantitative PCR to analyze gene expression in equine skin and sarcoids.
Participant Demographics
Samples were taken from 8 normal equine skin and 8 equine sarcoid samples.
Digital Object Identifier (DOI)
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