Effects of Lysine and Methionine on Gene Expression in Bovine Mammary Cells
Author Information
Author(s): Li Boning, Edick Ashlin M., Fox Madison K., Doelman John, Burgos Sergio A., Cant John P.
Primary Institution: Department of Animal Biosciences, University of Guelph, Ontario, Canada
Hypothesis
The deprivation of Lys or Met can inhibit protein synthesis, ER biogenesis, cell proliferation and lipogenesis through a chronic, long-term transcriptional response in bovine mammary epithelial cells.
Conclusion
EAA deficiency decreased the rate of protein synthesis and increased the expression of ATF4, indicating that the classic amino acid response pathway was triggered in bovine mammary epithelial cells.
Supporting Evidence
- EAA deficiency suppressed protein synthesis and activated ATF4 expression.
- Low lysine and methionine treatments decreased cell proliferation rates.
- Gene expression changes were observed after 40 hours of EAA deficiency.
Takeaway
When cows don't get enough of certain building blocks called amino acids, their milk-making cells don't work as well and make less milk.
Methodology
Bovine mammary epithelial cells were cultured in media with varying levels of lysine and methionine, and gene expression was measured using qPCR.
Limitations
The study was conducted in a cell culture model, which does not replicate in vivo conditions, and gene expression was measured only at the transcriptional level.
Participant Demographics
Bovine mammary epithelial cells from lactating Holstein cows.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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