Methylation of a Gene Related to Asparaginase Sensitivity in Lymphoma Cells
Author Information
Author(s): Peng H, Shen N, Qian L, Sun X-L, Koduru P, Goodwin L O, Issa J-P, Broome J D
Primary Institution: Department of Pathology, North Shore University Hospital, Manhasset, NY 11030
Hypothesis
The study investigates the relationship between hypermethylation of the asparagine synthetase gene and asparaginase sensitivity in lymphoma cells.
Conclusion
Hypermethylation of the asparagine synthetase gene is linked to the lack of mRNA expression in sensitive lymphoma cells.
Supporting Evidence
- Complete methylation of the CpG island in sensitive cells correlates with failure of mRNA expression.
- Resistant cells possess both methylated and unmethylated alleles.
- Normal splenic cells show age-related methylation patterns.
Takeaway
The study found that certain lymphoma cells that are sensitive to a drug have a gene that is completely turned off due to a chemical change, while resistant cells have both active and inactive versions of that gene.
Methodology
The promoter region of the murine asparagine synthetase gene was sequenced and its methylation profile was examined in different lymphoma cell lines.
Participant Demographics
The study involved murine (mouse) models, including adult mice and weanlings.
Digital Object Identifier (DOI)
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