A Sir2-Like Protein Participates in Mycobacterial NHEJ
2011
Sir2 Protein's Role in Mycobacterial DNA Repair
publication
Evidence: high
Author Information
Author(s): Li Zhongdao, Wen Jikai, Lin Yaning, Wang Shihua, Xue Peng, Zhang Zhiping, Zhou Ying, Wang Xiao, Sui Li, Bi Li-Jun, Zhang Xian-En
Primary Institution: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
Hypothesis
Sir2 is involved in the mycobacterial nonhomologous end-joining (NHEJ) repair pathway.
Conclusion
Sir2 plays a significant role in the efficiency of DNA double-strand break repair in mycobacteria.
Supporting Evidence
- Sir2 interacts directly with Ku and LigD in mycobacteria.
- The efficiency of DSB repair in a sir2 deletion mutant was reduced 2-fold.
- The Δsir2 strain was about 10-fold more sensitive to ionizing radiation in the stationary phase than the wild-type.
- Overexpression of Sir2 enhanced NHEJ activity.
Takeaway
Sir2 is a helper protein that helps bacteria fix broken DNA, making them stronger against damage.
Methodology
The study used tandem affinity purification and in vivo plasmid-based end-joining assays to investigate the role of Sir2 in NHEJ.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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