Activated Protein C and Vasoconstriction in Rabbit Shock Model
Author Information
Author(s): Wiel Eric, Costecalde Marion Elizabeth, Lebuffe Gilles, Corseaux Delphine, Jude Brigitte, Bordet Régis, Tavernier Benoît, Vallet Benoît
Primary Institution: University Hospital of Lille, France
Hypothesis
The study investigates the effects of activated protein C (aPC) on vascular function, endothelial injury, and haemostasis in a rabbit endotoxin-induced shock model.
Conclusion
Activated protein C increases sensitivity to a vasoconstrictor agent and protects against endothelial injury in endotoxin-induced shock.
Supporting Evidence
- LPS decreased sensitivity to phenylephrine in aortic rings without endothelium.
- aPC treatment restored endothelium-dependent sensitivity in response to phenylephrine.
- Endotoxin-induced de-endothelialisation was reduced by aPC treatment.
Takeaway
This study shows that aPC helps rabbits' blood vessels respond better to constriction and protects their blood vessel linings when they are in shock.
Methodology
The study involved 22 male New Zealand rabbits, with vascular reactivity and endothelial injury assessed after treatment with aPC and LPS.
Potential Biases
Potential bias due to the single bolus administration of aPC and the timing of its administration relative to LPS injection.
Limitations
The study did not assess plasma levels of aPC, and the effects on mortality were not conclusive due to low mortality rates.
Participant Demographics
22 male New Zealand rabbits, weighing 2.5 to 3 kg each.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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