CD34+ Cells as Functional Endothelial Progenitor Cells in Mice
Author Information
Author(s): Yang Junjie, Ii Masaaki, Kamei Naosuke, Alev Cantas, Kwon Sang-Mo, Kawamoto Atsuhiko, Akimaru Hiroshi, Masuda Haruchika, Sawa Yoshiki, Asahara Takayuki
Primary Institution: Group of Vascular Regeneration Research, Institute of Biomedical Research and Innovation/RIKEN Center for Developmental Biology, Kobe, Japan
Hypothesis
CD34 could be used for identification and isolation of functional EPCs from mouse bone marrow.
Conclusion
Mouse CD34+ cells may represent a functional EPC population in bone marrow, which could benefit the investigation of therapeutic EPC biology.
Supporting Evidence
- CD34+ cells showed the lowest EPC colony forming activity but exhibited the highest mRNA expression levels of endothelial markers.
- CD34+ cells demonstrated a dramatic increase in immediate recruitment to the myocardium after myocardial infarction.
- Cell retention and incorporation into the vasculature of the ischemic myocardium was significantly higher in the CD34+ cell-injected group.
Takeaway
Scientists found that a specific type of cell called CD34+ cells from mouse bone marrow can help heal the heart after injury by turning into blood vessel cells.
Methodology
CD34+ cells were isolated from mouse bone marrow using fluorescent activated cell sorting, and their properties were analyzed in vitro and in vivo.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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