How Schistosoma mansoni HMGB1 Protein is Regulated and Released
Author Information
Author(s): Isabel Caetano da Silva, Vitor Coutinho Maciel, Renata de Moraes da Costa, Rodrigo Furtado Madeiro, Daniel Rodrigues de Oliveira, Francisco Meirelles Bastos, Mário Alberto Cardoso Rumjanek, Franklin David Fantappié, Marcelo Rosado Bozza
Primary Institution: Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro
Hypothesis
How does the nuclear SmHMGB1 reach the extracellular space?
Conclusion
Secretion of SmHMGB1 is regulated by phosphorylation, suggesting it may act as a new egg antigen in schistosomiasis.
Supporting Evidence
- CK2 phosphorylation was shown to be involved in the nucleocytoplasmic shuttling of SmHMGB1.
- Phosphorylation did not affect the DNA binding activities of SmHMGB1.
- SmHMGB1 was found in both the nucleus and cytoplasm of adult schistosomes.
Takeaway
The study found that a protein from a parasite can move from the nucleus to outside the cell, and this movement is controlled by a process called phosphorylation.
Methodology
The study used in vitro and in vivo experiments to analyze the phosphorylation and cellular traffic of SmHMGB1.
Digital Object Identifier (DOI)
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