How CD28 Affects T Cell Activation
Author Information
Author(s): Raychaudhuri Kumarkrishna, Rangu Rohita, Ma Alison, Alvinez Neriah, Tran Andy D., Pallikkuth Sandeep, McIntire Katherine M., Garvey Joseph A., Yi Jason, Samelson Lawrence E.
Primary Institution: National Cancer Institute, National Institutes of Health
Hypothesis
CD28 co-stimulation lowers the TCR activation threshold by recruiting the activated form of Lck into the TCR microclusters.
Conclusion
CD28 co-stimulation enhances T cell receptor signaling by accelerating the recruitment of ZAP70 and altering Lck dynamics.
Supporting Evidence
- CD28 co-stimulation accelerates recruitment of ZAP70 to TCR microclusters.
- CD28 enhances the activated form of Lck in TCR microclusters.
- CD28 co-stimulation leads to earlier calcium flux in T cells.
Takeaway
When T cells get a signal from CD28, they can respond better and faster to other signals, helping them fight infections.
Methodology
TIRF microscopy was used to study the recruitment of signaling proteins in live T cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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