L-glutaminase from Streptomyces sp. strain 5 M: A potential anti-cancer enzyme
Author Information
Author(s): Hassan Mervat G., El-Sayyad Gharieb S., Abdel-Monem Mohamed O., Malash Mohamed N., Kishk Mona A., El Awady Mohamed E., El-khonezy Mohamed I.
Primary Institution: Department of Botany and Microbiology, Faculty of Science, Benha University, Benha, Egypt
Hypothesis
Can L-glutaminase produced by Streptomyces sp. strain 5 M exhibit anti-neoplastic activity?
Conclusion
Streptomyces sp. strain 5 M produces L-glutaminase, which shows significant anti-cancer properties.
Supporting Evidence
- Nine out of 34 Streptomyces isolates produced significant amounts of L-glutaminase.
- The enzyme showed strong toxicity against HepG-2, HeLa, and MCF-7 cancer cell lines.
- The optimal conditions for enzyme production were identified as pH 7.5 and 40 °C.
- The molecular weight of the enzyme was estimated to be 52 kDa.
- The Km and Vmax values for the enzyme were 2.62 mM and 10.20 U/ml, respectively.
Takeaway
Scientists found a special enzyme from a type of bacteria that can help fight cancer by stopping cancer cells from growing.
Methodology
The study involved isolating Streptomyces strains from various environments, testing for L-glutaminase production, and analyzing its properties and effects on cancer cell lines.
Limitations
The study's findings are based on in vitro tests and may not fully represent in vivo effects.
Digital Object Identifier (DOI)
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