Effect of 5/6 Nephrectomized Rat Serum on Epithelial-to-Mesenchymal Transition In Vitro
Author Information
Author(s): Lu Zhaoyu, Xie Yuansheng, Liu Xusheng, Cui Shaoyuan, Wu Yuchi, Cai Cun, Zhang Lei, Chen Xiangmei
Primary Institution: Chinese PLA General Hospital
Hypothesis
Does the serum from 5/6 nephrectomized rats induce epithelial-to-mesenchymal transition in renal tubular cells?
Conclusion
The serum from 5/6 nephrectomized rats significantly suppresses E-cadherin expression in HK-2 cells, indicating its role in promoting epithelial-to-mesenchymal transition.
Supporting Evidence
- E-cadherin expression decreased significantly in the remaining kidney at 12 weeks.
- 5/6Nx rats’ 12-week serum suppressed E-cadherin protein and mRNA expression.
- 5/6Nx rats’ serum upregulated ZEB1, β-catenin, and wnt3 protein expression.
Takeaway
The study shows that serum from rats with kidney surgery can change kidney cells in a way that might make them less effective, which could help us understand kidney disease better.
Methodology
24 Sprague Dawley rats were divided into two groups, and serum was collected after 12 weeks to assess its effect on HK-2 cells.
Limitations
The exact constituents of the 5/6Nx rats’ serum were not clarified.
Participant Demographics
Male Sprague Dawley rats, 3 months old.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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