Gene Expression Profiling of Dendritic Cells and Staphylococcus Infections
Author Information
Author(s): Toufeer Mehdi, Bonnefont Cécile M. D., Foulon Eliane, Caubet Cécile, Tasca Christian, Aurel Marie-Rose, Robert-Granié Christèle, Rupp Rachel, Foucras Gilles
Primary Institution: Université de Toulouse, INP, ENVT; UMR 1225, IHAP, Toulouse, France
Hypothesis
Dendritic cells from animals showing different degrees of susceptibility to staphylococcal infections may have distinct transcriptional profiles upon infection.
Conclusion
The distinct transcriptional profiles of dendritic cells obtained from resistant and susceptible animals may explain susceptibility towards S. aureus infections.
Supporting Evidence
- 204 genes were statistically differentially expressed between susceptible and resistant animals.
- Pro-inflammatory cytokines were significantly up-regulated in dendritic cells after stimulation.
- Distinct gene expression profiles were observed at different time points post-stimulation.
Takeaway
This study looked at how certain immune cells react to a bacteria called Staphylococcus aureus in sheep, finding that some sheep are better at fighting off the bacteria than others.
Methodology
The study used bone marrow-derived dendritic cells from sheep, stimulated them with heat-killed S. aureus, and analyzed gene expression using microarrays.
Limitations
The study was limited to a specific breed of sheep and may not be generalizable to other breeds or species.
Participant Demographics
Two-year old ewes (Lacaune breed) from resistant and susceptible lines.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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