Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle
Author Information
Author(s): Chiara Donati, Giuseppina Marseglia, Alberto Magi, Simona Serratì, Francesca Cencetti, Caterina Bernacchioni, Genni Nannetti, Matteo Benelli, Silvia Brunelli, Francesca Torricelli, Giulio Cossu, Paola Bruni
Primary Institution: Dipartimento di Scienze Biochimiche, Università di Firenze, Firenze, Italia
Hypothesis
The study investigates the role of sphingosine 1-phosphate (S1P) in promoting the differentiation of mesoangioblasts into smooth muscle cells.
Conclusion
S1P promotes the differentiation of mesoangioblasts into smooth muscle cells by up-regulating the transcription factors GATA6 and LMCD1.
Supporting Evidence
- S1P was shown to enhance the expression of smooth muscle-specific proteins.
- GATA6 and LMCD1 were identified as key transcription factors in the differentiation process.
- Pharmacological inhibition of S1P formation abrogated GATA6 up-regulation.
- S1P treatment resulted in significant morphological changes in mesoangioblasts.
Takeaway
This study shows that a special fat called S1P helps certain stem cells turn into muscle cells that are important for blood vessels.
Methodology
The study used cDNA microarray analysis, quantitative mRNA and protein analysis, and gene silencing experiments to investigate the effects of S1P on mesoangioblast differentiation.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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