Real-Time Measurement of Solute Transport Within the Lacunar-Canalicular System of Mechanically Loaded Bone: Direct Evidence for Load-Induced Fluid Flow
2011
Real-Time Measurement of Fluid Flow in Bone
Sample size: 13
publication
Evidence: high
Author Information
Author(s): Christopher Price, Xiaozhou Zhou, Wen Li, Liyun Wang
Primary Institution: University of Delaware
Hypothesis
Can mechanical loading enhance solute transport in the bone lacunar-canalicular system?
Conclusion
Mechanical loading significantly enhances the transport of sodium fluorescein in the bone lacunar-canalicular system.
Supporting Evidence
- Fluorescein transport increased by 31% under mechanical loading.
- Peak canalicular fluid velocity was estimated at 60 µm/s.
- Statistical analysis showed significant differences in transport rates.
Takeaway
When bones are squeezed, they can move fluids better, which helps cells get the nutrients they need.
Methodology
The study used fluorescence recovery after photobleaching (FRAP) to measure solute transport in loaded and nonloaded bone.
Limitations
The study used ex vivo samples, which may not fully represent in vivo conditions.
Participant Demographics
Adult male C57BL/6J mice, aged 12-16 weeks.
Statistical Information
P-Value
<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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